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KMID : 0608520070130010147
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Korean Journal of Oriental Medicine
2007 Volume.13 No. 1 p.147 ~ p.152
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Discrimination of Phlomidis Radix and Dipsaci Radix using the Random Amplified Polymorphic DNA Analysis
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Lee Mee-Young
Ryuk Jin-Ah
Kim Hong-Jun
Kim Young-Hwa
Chae Byoung-Chan
Ko Byoung-Seob
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Abstract
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As a result to amplifying 12 samples of "Sok-dan" through an random amplified polymorphic DNA (RAPD) method using eighteen UBC and URP primers, distinct band forms enabling discrimination of Phlomus umbrosa and Dipsacus asperoides were observable in the UBC 320 primer, UBC 367 primer, UBC 385 primer, UBC 414 primer, UBC 423 primer, URP 3 primer, URP 5 primer and URP 9 primer. The polymorph result amplified with a random primer was evaluated through Gelcompar Ⅱ, showing a result dividable into two groups. The divided groups were the dried sample group of Dipsacus asperoides and the group of Phlomis umbrosa. In order to recognize the distinction between Dipsaci Radix types, the genetic variation of "Sok-dan" produced domestically and imported was evaluated through RAPD, and the potential to distinguish these in forms of dried medicine was identified, presenting a method to authentification of Phlomis umbrosa and Dispacus asperoides.
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KEYWORD
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Dipsaci Radix, Phlomidis Radix, discrimination, random amplified polymorphic DNA(RAPD), genetic analysis
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